Bacterial Cell Breakage or Lysis

Authored by: Matthew E. Bahamonde

Practical Handbook of Microbiology

Print publication date:  June  2015
Online publication date:  June  2015

Print ISBN: 9781466587397
eBook ISBN: 9781466587403
Adobe ISBN:


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While bacterial cell lysis is a relatively straightforward process, there are significant variations in its practice. The type of lysis is often dependent upon what subcellular component is to be purified such as the isolation of DNA versus protein, the scale of the project; miniprep versus industrial scale, and the type of microbe; and gram-negative versus gram-positive. Generally, microbial lysis methods can be broken down into three categories based on the relative harshness/intensity of the method: gentle, moderate, or vigorous (see Table 16.1, e.g., of each). 1 Many of these methods give comparable results, and only personal preference plays a role in selection. Others are better suited to specific tasks and should be looked on with preference.

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