Fluorescent Proteins: Structural Determinants of Optical Response

Authored by: Ranieri Bizzarri , Riccardo Nifosì

CRC Handbook of Organic Photochemistry and Photobiology

Print publication date:  March  2012
Online publication date:  March  2012

Print ISBN: 9781439899335
eBook ISBN: 9781466561250
Adobe ISBN:


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The discovery of a fluorescent protein (FP) taking part in the bioluminescence system of jellyfish Aequorea victoria dates back to the early 1960s [1]. This protein was named green fluorescent protein (GFP), from its bright green fluorescence obtained by UV–blue light excitation; hereafter, it will be simply referred to as wild-type GFP or wtGFP to be consistent with the scientific nomenclature in use.

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