DNA Sequencing and Analysis

Authored by: Leland J. Cseke , Larka Lance

Handbook of Molecular and Cellular Methods in Biology and Medicine

Print publication date:  December  2011
Online publication date:  April  2016

Print ISBN: 9781420069389
eBook ISBN: 9781439881958
Adobe ISBN:

10.1201/b11351-9

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Abstract

DNA sequencing is a necessary technique for almost all molecular biology studies including DNA cloning, characterization, mutagenesis, DNA recombination, regulation of gene expression, 1 3 and protein expression. There are several well-established methods for nucleic acid sequencing, and in recent years, advances in automated sequencing have made DNA sequence analysis an easy and routine process. The present chapter describes in detail the preparation of DNA for sequencing. 4 6 DNA sequencing by dideoxynucleotides chain termination2,3 and cycle sequencing. 1 , 4 6 These techniques are the foundation of most sequencing applications; however, with the advent of automated sequencing and the availability of automated liquid handlers, we also briefly describe how the newer machines and sources have greatly increased the speed of DNA sequence analysis. The following techniques are routinely used in our respective labs.

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