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Genomic DNA Libraries

Authored by: Leland J. Cseke , Kirakosyan Ara , Peter B. Kaufman

Handbook of Molecular and Cellular Methods in Biology and Medicine

Print publication date:  December  2011
Online publication date:  April  2016

Print ISBN: 9781420069389
eBook ISBN: 9781439881958
Adobe ISBN:

10.1201/b11351-8

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Abstract

Genomic DNA cloning is a technology that plays an important role in state-of-the-art molecular biology studies. Many of the genes that have been characterized originally come from genomic DNA cloning. A genomic clone contains much more information than a cDNA clone because it possesses regulatory regions such as promoter and terminator regions, intron sequences that may also be involved in regulation and overall gene structure, and in some cases methylation information that may be responsible for chromatin structure. If one wishes to identify, characterize, and regulate the expression of a full-length unknown genomic gene, he or she may have to start from molecular cloning of genomic DNA, which is described in detail in this chapter.

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