Laser Capture Microdissection and Whole Genome Amplification

Authored by: Leland J. Cseke , Kirakosyan Ara , Peter B. Kaufman , Margaret V. Westfall , Ramesh Buyyarapu , Venkateswara Rao Sripathi , Sarah Beth Cseke , Ramesh V. Kantety

Handbook of Molecular and Cellular Methods in Biology and Medicine

Print publication date:  December  2011
Online publication date:  April  2016

Print ISBN: 9781420069389
eBook ISBN: 9781439881958
Adobe ISBN:

10.1201/b11351-47

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Abstract

Understanding of cellular and molecular interactions as well as gene expression patterns that lead to a tissue condition necessitates extraction of homogeneous subpopulations or even single cell for further analysis. Cell sorting using flow cytometry and affinity-labeled magnetic beads require prior dissolution of intercellular adhesion to allow separation of subpopulations from heterogeneous cell suspension. However, these techniques limit the extraction of intact tissue mass that may be critical in some instances for visual observations. Microdissection procedure originally involved manual guidance of needle to scrape and recover target region from thin tissue film that was highly tedious, inaccurate, and operator dependent. 1 To overcome these disadvantages, laser capture and microdissection (LCM) was initially developed at the National Institutes of Health (NIH) for the selective isolation of human cell populations from a section of complex, heterogeneous tissue with the help of carbon dioxide laser that pulses on targeted cells coated with a thermoplastic film and causes strong focal adhesion. 2

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