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Plant Tissue and Cell Culture

Authored by: Nidhi P. Chanana , Leland J. Cseke , Ara Kirakosyan , Peter B. Kaufman

Handbook of Molecular and Cellular Methods in Biology and Medicine

Print publication date:  December  2011
Online publication date:  April  2016

Print ISBN: 9781420069389
eBook ISBN: 9781439881958
Adobe ISBN:

10.1201/b11351-42

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Abstract

Success in plant molecular biology and biotechnology is largely dependent on in vitro culture of plant cells and tissues. 1 9 Transgenic crop technology offers an opportunity to scrutinize the in vivo function of particular gene products and to develop new cultivars having novel genetic traits. Genetically modified plants that contain foreign genes have been extensively developed using plant tissue culture techniques. Globally, around one billion ha of land was under cultivation of transgenic crops in 2010 for commercial as well as research purposes. Potential use of in vitro plant cell culture for producing commercially valuable products has also been studied extensively. In the last decade, there has been a large increase in the number of plant species successfully cultured, either as cell suspensions or as hairy root cultures, for the production of secondary metabolites. Besides, there are diverse examples of plant tissue and cell cultures that have been used in crop improvement for disease resistance, salt tolerance, cold tolerance, drought tolerance, and herbicide resistance.

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