DNA Cloning Strategies

Authored by: Leland J. Cseke , Geetika Trivedi , Avinash Sreedasyam , Peter B. Kaufman , Ara Kirakosyan

Handbook of Molecular and Cellular Methods in Biology and Medicine

Print publication date:  December  2011
Online publication date:  April  2016

Print ISBN: 9781420069389
eBook ISBN: 9781439881958
Adobe ISBN:

10.1201/b11351-4

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Abstract

DNA Cloning is the process of moving a gene from the chromosome it occurs in naturally to an autonomously replicating vector. In the cloning process, the DNA is removed from cells, manipulations of the DNA are carried out in a test tube, and the DNA is subsequently put back into cells. Because Escherichia coli is so well characterized, it is usually the cell of choice for manipulating DNA molecules. Once the appropriate combination of vector and cloned DNA or construct has been made in E. coli, the construct can then be inserted into other cell types.

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