Isolation and Purification of DNA

Authored by: Leland J. Cseke , Chuanfu An , Chung-Jui Tsai

Handbook of Molecular and Cellular Methods in Biology and Medicine

Print publication date:  December  2011
Online publication date:  April  2016

Print ISBN: 9781420069389
eBook ISBN: 9781439881958
Adobe ISBN:

10.1201/b11351-3

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Abstract

Deoxyribonucleic acid (DNA) isolation and purification are essential techniques for molecular biology studies. The quality and integrity of the DNA isolated directly affects the results of all subsequent scientific research. 1 4 A number of methods have been developed for extraction and purification of DNA from different tissue types and organisms. We present several well-developed protocols here for the isolation of DNA from bacteria, lambda phages, animals, and plants and the purification of DNA fragments from agarose gels.

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