Isolation and Purification of DNA

Authored by: Leland J. Cseke , Chuanfu An , Chung-Jui Tsai

Handbook of Molecular and Cellular Methods in Biology and Medicine

Print publication date:  December  2011
Online publication date:  April  2016

Print ISBN: 9781420069389
eBook ISBN: 9781439881958
Adobe ISBN:


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Deoxyribonucleic acid (DNA) isolation and purification are essential techniques for molecular biology studies. The quality and integrity of the DNA isolated directly affects the results of all subsequent scientific research. 1 4 A number of methods have been developed for extraction and purification of DNA from different tissue types and organisms. We present several well-developed protocols here for the isolation of DNA from bacteria, lambda phages, animals, and plants and the purification of DNA fragments from agarose gels.

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