Localization of Protein Expression

Authored by: Ara Kirakosyan , Peter B. Kaufman , Margaret V. Westfall , Michael Mashore , Casey R. Lu , E. Mitchell Seymour

Handbook of Molecular and Cellular Methods in Biology and Medicine

Print publication date:  December  2011
Online publication date:  April  2016

Print ISBN: 9781420069389
eBook ISBN: 9781439881958
Adobe ISBN:

10.1201/b11351-21

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Abstract

Once proteins are synthesized on ribosomes via the process of translation, specific kinds of proteins become targeted for localization in the nucleus; in organelles that include plastids, Golgi, vacuoles, mitochondria, lysosomes; in cytoskeleton structures such as microtubules, actin filaments, and microfilaments; in membrane systems such as rough and smooth endoplasmic reticulum, Golgi vesicles, vacuolar membranes, the nuclear membrane, and the plasma membrane; in cell walls and plasmodesmata of plant cells; in cilia and flagellae; and as coat proteins of viruses. We can carry this analysis a step further in order to search for the specific kinds of proteins localized at these marker sites. To get started, go to this website: http://www.genecopoeia.com/product/search/organelle_marker/.

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