Preparation of RNA Probes

Authored by: Peter B. Kaufman , Leland J. Cseke , Kirakosyan Ara , Avinash Sreedasyam , Trivedi Geetika

Handbook of Molecular and Cellular Methods in Biology and Medicine

Print publication date:  December  2011
Online publication date:  April  2016

Print ISBN: 9781420069389
eBook ISBN: 9781439881958
Adobe ISBN:

10.1201/b11351-13

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Abstract

RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA, is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, and transcription. Whereas DNA probes are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or sub-species, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I or non-radioactive moieties such as biotin and digoxigenin (DIG).

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