Dual Axes Confocal Microscopy

Authored by: Michael J. Mandella , Thomas D. Wang

Handbook of Photonics for Biomedical Science

Print publication date:  May  2010
Online publication date:  May  2010

Print ISBN: 9781439806289
eBook ISBN: 9781439806296
Adobe ISBN:

10.1201/9781439806296-c19

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Abstract

This chapter describes the theory, design, and implementation of a novel confocal architecture that uses separate optical axes for illumination and collection of light. This configuration can achieve subcellular resolution (< 5 μm) with a deep tissue penetration (> 500 μm) and a high dynamic range. Moreover, a large field-of-view can be generated with a diffraction-limited focal volume using post-objective scanning that also allows for this configuration to be scaled down in size to millimeter dimensions without loss of performance. Endoscope compatibility can be achieved with use of a MEMS (micro-electro-mechanical systems) scanner and fiber coupling. An instrument of this size can be used to perform real-time histopathology from the epithelium of hollow organs and longitudinal studies in small animals.

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