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Experiments

Authored by: L. Eggeling , O. Reyes

Handbook of Corynebacterium glutamicum

Print publication date:  March  2005
Online publication date:  March  2005

Print ISBN: 9780849318214
eBook ISBN: 9781420039696
Adobe ISBN:

10.1201/9781420039696.pt7

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Abstract

This chapter presents a selection of protocols for experimental studies with C. glutamicum as well as an overview on plasmid vectors described for this species. The first two protocols describe alternative methods for eliciting glutamate excretion, the name-giving property of C. glutamicum. The protocols focus on basic molecular genetic techniques, which are essential prerequisites for metabolic engineering and a diversity of other studies. Most experiments can be performed within a few days except those describing targeted gene deletion and transposon mutagenesis, which require at least two weeks. Prerequisites for the successful performance of the experiments are a basic knowledge of microbiological and molecular genetic techniques, in particular of the principles of sterile handling, and basic equipment as available in microbiological laboratories and for student courses.

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