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Handbook of Cryo-Preparation Methods for Electron Microscopy

Edited by: Annie Cavalier , Danièle Spehner , Bruno M. Humbel

Print publication date:  October  2008
Online publication date:  October  2008

Print ISBN: 9780849372278
eBook ISBN: 9781420006735
Adobe ISBN:

10.1201/9781420006735
 Cite  Marc Record

Book description

While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable.

The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations.

Contributions to this handbook –

Ö         Describe cryo-methods aimed at perfect preservation for fine structural analysis

Ö         Teach how to arrest physiological processes by cryo-fixation

Ö         Reveal the secrets for high-resolution snapshots of life by CEMOVIS

Ö         Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography

Ö         Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules

Ö         Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography

Ö         Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding

Ö         Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling

Ö         Include summary tables to compare and obtain appropriate criteria

Includes a wealth of immediate reference material for daily use

With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.

Table of contents

Chapter  1:  Vitreous Water Download PDF
Chapter  2:  Slam-Freezing, Metal-Mirror Freezing Download PDF
Chapter  3:  Plunge-Freezing (Holey Carbon Method) Download PDF
Chapter  4:  Controlled Vitrification Download PDF
Chapter  5:  BAL-TEC HPM 010 High-Pressure Freezing Machine Download PDF
Chapter  6:  High-Pressure Freezing Leica Empact Download PDF
Chapter  7:  Frozen Hydrated Macromolecules for Structural Analysis Download PDF
Chapter  8:  Two-Dimensional Crystals Download PDF
Chapter  9:  Cryo-Negative Staining Download PDF
Chapter  10:  Vitrification of Dynamic Microtubules Download PDF
Chapter  11:  Cemovis, Cryo-Electron Microscopy of Vitreous Sections Download PDF
Chapter  12:  Cryo- Electron Tomography Download PDF
Chapter  13:  Freeze-Substitution Download PDF
Chapter  14:  Cryo-Fixation, Freeze-Substitution, Rehydration and Tokuyasu Cryo-Sectioning Download PDF
Chapter  15:  Freeze-Drying and Embedding of Biological Material Download PDF
Chapter  16:  The Shadow of Hydrated Biological Specimens Download PDF
Chapter  17:  Cryo-Fracture of Self Assembled Systems in Organic Solvents Download PDF
Chapter  18:  Progressive Lowering of Temperature for Immunolabeling and in situ Hybridization Download PDF
Chapter  19:  Cryo-Sectioning According to Tokuyasu Download PDF
Chapter  20:  Cryo-Preparation Procedures for Elemental Imaging by Sims and Eftem Download PDF
Chapter  21:  Correlative Light and Electron Microscopy Download PDF
Chapter  22:  SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL) Download PDF
Chapter  23:  Immunolabeling of Ultra-Thin Sections with Enlarged 1 nm Gold or Qdots Download PDF
Chapter  24:  3-D Electron Tomography of Cells and Organelles Download PDF
Glossary Download PDF
prelims Download PDF
Final Considerations Download PDF
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