Production of Lignin Peroxidase by Phanerochaete chrysosporium Immobilized on Porous Poly(styrene-divinylbenzene) Carrier and Its Application to the Degrading of 2-Chlorophenol

Authored by: Eli Ruckenstein , Xiao-Bai Wang

Concentrated Emulsion Polymerization

Print publication date:  April  2019
Online publication date:  March  2019

Print ISBN: 9780367134556
eBook ISBN: 9780429026577
Adobe ISBN:


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Porous poly(styrene-divinylbenzene) carriers for the immobilization of white rot fungus Phanerochaete chrysosporium have been prepared by the concentrated emulsion polymerization method. The concentrated emulsion consists of a mixture of styrene and divinylbenzene containing a suitable surfactant and an initiator as the continuous phase, and water as the dispersed phase. The polymerization of the monomers of the continuous phase generated the polymer carrier with a porous structure. The white rot fungus Phanerochaete chrysosporium has been immobilized on porous poly(styrene-divinylbenzene) carriers and used for the batch production and the repeated batch production of lignin peroxidase in shake cultures based on a carbon-limited medium containing veratryl alcohol. The best results were achieved when a spore inoculum was used for immobilization instead of 1-day-old mycelial pellets, for both the batch production and the repeated batch production. The porous poly(styrene-divinylbenzene) immobilized Phanerochaete chrysosporium and freely suspended mycelial pellets were used as biocatalysts for the degradation of 2-chlorophenol in a 2-L bioreactor. The porous poly(styrene-divinylbenzene) particle (diameter ≅ 0.2 cm) immobilized spores exhibited a much higher activity in the degradation of 2-chlorophenol than the freely suspended mycelial pellets. © 1994 John Wiley & Sons.

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