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In This Chapter
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Spectroscopic Methods of Analysis: Fluorescence Spectroscopy
- Introduction
- Molecular Energy Levels and Electronic Processes
- Characteristics of Luminescence Spectra
- Fluorescence
- Quantum Yield and Decay-Time of Fluorescence
- Chemical Structural Aspects of Fluorescence Spectra
- Solvent Effects
- pH Effects
- Quenching
- Concentration Effects
- Temperature Effects
- Current Trend in Fluorescence Techniques
- Fluorescence Resonance Energy Transfer
- Fluorescence Anisotropy
- Time-Resolved Fluorescence Technique
- Fluorescence Correlation Spectroscopy
- Multiphoton Fluorescence Spectroscopy
- Pharmaceutical Applications of Fluorescence Techniques
- Drug Discovery Stage—Target Validation, In Vitro and In Vivo Activity Assays
- Research and Development Stage—Analytical and Bioanalytical Applications
- Laser-Induced Fluorescence (LIF) Detection in Separation Methods
- Fluoroimmunoassay
- Lif As A “New” Tool for Pharmaceutical Process Analytical Technology (Pat)
- Sirna Encapsulation Efficiency
- Fluorescence Application in Toxicology Studies
- Articles of Further Interest
- References
Abstract
Luminescence processes in molecules can be classified according to the source from where the excitation energy is derived. In photoluminescence, which encompasses fluorescence and phosphorescence, excitation is achieved by the absorption of light by the potentially luminescent molecule. In chemiluminescence, excitation occurs as the result of an energetic chemical reaction.
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